Applications of sorbent extraction in pharma analysis are numerous, since they allow simplification and acceleration of assays, previously carried out by HPLC. Recent comprehensive review (Solich 2003), along with applications outlined here, highlight the features of this powerful methodology.
SPE microcolumn (Huclova 2006), was used for selective retention of amiloride, while the urine matrix components were eluted with water carrier flow to the waste. Due to the acid-basic and polarity properties of amiloride molecule on the ion-exchange sorbent (SPE BAKER WCX carboxy group), it was feasible to retain analyte molecules on the microcolumn, while urine matrix components were washed out. After elution with 0.01M HNO3 + 0.1M KCl, the analyte was detected by fluorescence 385 nm. Recovery was found in the range 96.8–99.4% for 10 times diluted urine, linearity of determination in the range 0.5–100 gml-1 (r = 0.998), and 3s limit of detection (LOD) was 0.05 gml-1.
Sorbent Extraction for Pharmaceutical Assays
2.3.24.
